职称：教授

学位：博士

政治面貌：中共党员

邮箱：tujiagang@mail.hzau.edu.cn

研究方向：鱼类病毒致病机理及免疫防控

教育经历

2002.09-2006.06 bat365官网登录在线主页生命科学技术学院，获学士学位

2006.09-2011.12 bat365官网登录在线主页动物科学技术学院动物医学院，获博士学位

主要职历

2012.09-2015.05 美国德州大学休斯敦健康科学中心, 博士后

2015.10-2017.09 bat365手机版app，团队博士后

2017.10-2023.10 bat365手机版app，副教授，硕士生导师

2021.12至今 bat365手机版app，博士生导师

2023.11至今 bat365手机版app，教授

科研成果

主持科研项目

1. 国家重点研发计划子课题，大宗淡水鱼类出血病的发生机制及传播阻断技术研究，2024.01-2027.12，42万，在研

2. 国家自然科学基金面上项目，聚合酶复合物L-P通过泛素化修饰调节乌鳢水泡病毒增殖和毒力的分子机制研究，2022.01-2025.12，58万，在研

3. 国家自然科学基金面上项目，Leader RNA与N蛋白互作调节乌鳢水泡病毒增殖的分子机制研究，2020.01-2023.12，58万，已结题

4. 横向项目，鱼类病毒病疫苗和药物研发，2020.01-2022.12，60万，已结题

5. 国家自然科学基金青年项目，MiR-214在乌鳢水泡病毒感染SSN-1细胞中的调控机制研究，2017.01-2019.12，20万，已结题

6. 中央高校科研业务费，乌鳢水泡病毒P蛋白异构体P3的功能研究， 2018.01-2020.12，20万，已结题

7. 横向项目，乌鳢水泡病毒减毒疫苗的研制，2019.01-2019.12，10万，已结题

8. 中国博士后科学基金，乌鳢水泡病毒调控宿主microRNA的机制研究， 2017.01-2018.12，8万，已结题

发表科研论文

1. Chu Zhang, Hui Wu, Hao Feng, Yong-An Zhang*, Jiagang Tu*. Grass carp reovirus VP56 and VP35 induce formation of viral inclusion bodies for replication. iScience, 2024.

2. Xiangmou Qin, Ningyan Jiang, Jingjing Zhu, Yong-An Zhang*, Jiagang Tu*. Snakehead vesiculovirus hijacks SH3RF1 for replication via mediating K63-linked ubiquitination at K264 of the phosphoprotein. International Journal of Biological Macromolecules, 2024, 255:128201.

3. Xiangmou Qin, Yong-An Zhang*, Jiagang Tu*. P38MAPK- and GSK3-mediated phosphorylation of snakehead vesiculovirus phosphoprotein at threonine 160 facilitates viral replication. Journal of Virology, 2023, 97(5): e0040423.

4. An-Qi Liu, Xiangmou Qin, Hui Wu, Hao Feng, Yong-An Zhang*, Jiagang Tu*. hnRNPA1 impedes snakehead vesiculovirus replication via competitively disrupting viral phosphoprotein-nucleoprotein interaction and degrading viral phosphoprotein. Virulence, 2023, 14(1): 2196847.

5. Congran Bei^#, Chu Zhang^#, Hui Wu, Hao Feng, Yong-An Zhang, Jiagang Tu*. DDX3X is hijacked by snakehead vesiculovirus phosphoprotein to facilitate virus replication via stabilization of the phosphoprotein. Journal of Virology, 2023, 97(2): e0003523.

6. Chi Zhang, An-Qi Liu, Chu Zhang, Lan-Hao Liu, Jianguo Su, Yong-An Zhang*, Jiagang Tu*. MicroRNA miR-722 inhibits cyprinid herpesvirus 3 replication via targeting the viral immune evasion protein ORF89, which negatively regulates IFN by degrading IRF3. Journal of Immunology, 2022, 209(10):1918-1929.

7. Lin Jiang, An-Qi Liu, Chu Zhang, Yong-An Zhang*, Jiagang Tu*. Hsp90 regulates GCRV-II proliferation by interacting with VP35 as its receptor and chaperone. Journal of Virology, 2022, 96(19): e0117522.

8. Chi Zhang, Qing Wang, An-Qi Liu, Chu Zhang, Lan-Hao Liu, Long-Feng Lu, Jiagang Tu*, Yong-An Zhang*. MicroRNA miR-155 inhibits cyprinid herpesvirus 3 replication via regulating AMPK-MAVS-IFN axis. Developmental and Comparative Immunology, 2022, 129: 104335.

9. Yanwei Zhang, Yong-An Zhang*, Jiagang Tu*. Hsp90 is required for snakehead vesiculovirus replication via stabilization of the viral L protein. Journal of Virology, 2021, 95(16): e0059421.

10. Xiangmou Qin^#, Shuangshuang Feng^#, Yanwei Zhang, Jianguo Su, Li Lin, Yong-An Zhang*, Jiagang Tu*. Leader RNA regulates snakehead vesiculovirus replication via interacting with viral nucleoprotein. RNA Biology, 2021, 18(4): 537-546.

11. Yu Fu, Yong-An Zhang, Jinyu Shen*, Jiagang Tu*. Immunogenicity study of OmpU subunit vaccine against Vibrio mimicus in yellow catfish, Pelteobagrus fulvidraco. Fish and shellfish immunology, 2021, 108: 80-85.

12. Chi Zhang, Jiagang Tu*, Yong-An Zhang*. MicroRNA regulation of viral replication in teleost fish: a review. Reviews in Aquaculture, 2021, 13(3): 1367-1378.

13. Shuangshuang Feng, Jianguo Su, Li Lin*, Jiagang Tu*. Development of a reverse genetics system for snakehead vesiculovirus (SHVV), Virology, 2019, 526: 32-37.

14. Chi Zhang, Ningqiu Li, Xiaozhe Fu, Qiang Lin, Li Lin*, Jiagang Tu*. MiR-214 inhibits snakehead vesiculovirus (SHVV) replication by targeting host GS. Fish and shellfish immunology, 2019, 84:299-303.

15. Chunyan Wang#, Jiagang Tu#, Jun Liu*, Ian J. Molineux*. Structural dynamics of bacteriophage P22 infection initiation revealed by Cryo-electron tomography. Nature Microbiology, 2019, 4(6): 1049-56.

16. Chi Zhang, Shuangshuang Feng, Wenting Zhang, Nan Chen, Abeer M. Hegazy, Wenjie Chen, Xueqin Liu, Lijuan Zhao, Jun Li, Li Lin*, Jiagang Tu*. MicroRNA miR-214 inhibits snakehead vesiculovirus replication by promoting IFN-a expression via targeting host adenosine 5’-monophosphate-activated protein. Frontiers in immunology, 2017, 8:1775.

17. Chi Zhang, Lizhu Yi, Shuangshuang Feng. Xueqin Liu, Jianguo Su, Li Lin, Jiagang Tu*. MicroRNA miR-214 inhibits snakehead vesiculovirus (SHVV) replication by targeting the coding regions of viral N and P. Journal of General Virology, 2017, 98(7): 1611-1619.

18. Wenjie Chen, Lizhu Yi, Shuangshuang Feng, Lijuan Zhao, Jun Li, Meng Zhou, Rishen Liang, Na Gu, Zaohe Wu, Jiagang Tu*, Li Lin*. Characterization of microRNAs in orange-spotted grouper (Epinephelus coioides) fin cells upon red-spotted grouper nervous necrosis virus infection. Fish and shellfish immunology, 2017, 63:228-236.

19. Wenjie Chen, Lizhu Yi, Shuangshuang Feng, Xiaodan Liu, Muhammad Asim, Yongcan Zhou, Jiangfeng Lan, Sanjie Jiang, Jiagang Tu*, Li Lin*. Transcriptomic profiles of striped snakehead fish cells (SSN-1) infected with red-spotted grouper nervous necrosis virus (RGNNV) with an emphasis on apoptosis pathway. Fish and shellfish immunology, 2017, 60:346-354.

20. Muhammad Asim, Sanjie Jiang, Lizhu Yi, Wenjie Chen, Lindan Sun, Lijuan Zhao, Muhammad Nasir Khan Khattak, Jiagang Tu*, Li Lin*. Glutamine is required for red-spotted grouper nervous necrosis virus replication via replenishing the tricarboxylic acid cycle. Virus Research, 2017, 227:245-248.

21. Jiagang Tu, Changxu Tian, Peiqi Zhao, Junxiao Sun, Min Wang, Qixue Fan, Yongchao Yuan*. Identification and profiling of growth-related microRNAs in Chinese perch (Siniperca chuatsi). BMC Genomics, 2017, 18(1): 489.

22. Jiagang Tu, Taehyun Park, Dusustin R. Morado, Kelly T. Hughes, Ian J. Molineux*, Jun Liu*. Dual host specificity of phage SP6 is facilitated by tailspike rotation. Virology, 2017, 507:206-215.

23. Lindan Sun^#, Jiagang Tu^#, Lizhu Yi, Wenjie Chen, Lijuan Zhao, Yunmao Huang, Rishen Liang, Jun Li, Meng Zhou*, Li Lin*. Pathogenicity of snakehead vesiculovirus in rice field eels (Monopterus albus). Microbial Pathogenesis, 2017, 110:578-585.

24. Madeline M. Farley^#, Jiagang Tu^#, Daniel B. Kearns, Ian J. Molineux*, Jun Liu*. Ultrastructural analysis of bacteriophage Φ29 during infection of Bacillus subtilis. Journal of Structural Biology, 2017, 197 (2): 163-171.

25. Farman Ullah Dawar^#, Jiagang Tu^#, Muhammad Nasir Khan Khattak, Jie Mei*, Li Lin*. Cyclophilin A: A Key Factor in Virus Replication and Potential Target for Anti-viral Therapy. Current Issues in Molecular Biology, 2017, 21:1-20.

26. Lindan Sun, Lizhu Yi, Chi Zhang, Xiaodan Liu, Shuangshuang Feng, Wenjie Chen, Jiangfeng Lan, Lijuan Zhao, Jiagang Tu*, Li Lin*. Glutamine was required for snakehead fish vesiculovirus (SHVV) propagation via replenishing the tricarboxylic acid cycle. Journal of General Virology, 2016, 97(11): 2849-2855.

27. Jiagang Tu^#, Wenjie Chen^#, Xiaozhe Fu, Qiang Lin, Ouqin Chang, Lijuan Zhao. Jiangfeng Lan*, Ningqiu Li*, Li Lin*. Susceptibility of Chinese Perch Brain (CPB) Cell and Mandarin Fish to Red-Spotted Grouper Nervous Necrosis Virus (RGNNV) Infection. Int. J. Mol. Sci., 2016, 17(5): 740.

28. Xiaodan Liu^#, Jiagang Tu^#*, Junfa Yuan, Xueqin Liu, Lijuan Zhao, Farman Dawar. Muhammad Khattak, Abeer Hegazy, Nan Chen, Vikram Vakharia, Li Lin*. Identification and Characterization of MicroRNAs in Snakehead Fish Cell Line upon Snakehead Fish Vesiculovirus Infection. Int. J. Mol. Sci., 2016, 17(2): 154.

29. Lijuan Zhao^#, Jiagang Tu^#, Yulei Zhang, Jinfu Wang, Ling Yang, Weimin Wang, Zaohe Wu, Qinglei Meng*, Li Lin*. Transcriptomic analysis of the head kidney of Topmouth culter (Culter alburnus) infected with Flavobacterium columnare with an emphasis on phagosome pathway. Fish and Shellfish Immunology, 2016, 57:413-418.

30. Jiagang Tu^#, Jing Guo^#, Anding Zhang^#, Wenting Zhang, Zongzheng Zhao, Hongbo Zhou, Cheng Liu, Huanchun Chen, Meilin Jin*. Effects of the C-terminal truncation in NS1 protein of the 2009 pandemic H1N1 influenza virus on host gene expression. PLos One, 2011, 6(10): e26175.

31. Jiagang Tu^#, Hongbo Zhou^#, Chun Li, Anding Zhang, Xuebo Guo, Wei Zou, Huanchun Chen, Meilin Jin*. Isolation and molecular characterization of equine H3N8 influenza viruses from pigs in China. Arch Virol, 2009, 154(5): 887-890.

32. 伊丽竹，徐镇，林蠡，涂加钢*. miRNA在乌鳢水泡病毒感染月鳢细胞（SSN-1）中的表达变化及其调控作用. 水产学报. 2019, 43(5): 1288-1297.

33. 秘琮然，张永安，涂加钢*. 乌鳢水泡病毒P蛋白异构体的鉴定及其功能初探. 水产学报. 2021.

授权发明专利

1. 涂加钢，张永安。一种构建赤点石斑鱼神经坏死病毒反向遗传系统的方法。专利号: ZL202010239788.7; 授权公告日: 2022.03.08

2. 金梅林，涂加钢，周红波，徐晓娟，陈焕春。一种重组H5N1禽流感细胞苗及应用。专利号: ZL201010620151.9; 授权公告日: 2013.12.30

备注

入选国家级青年人才项目

研究方向：针对草鱼出血病、乌鳢水泡病毒病、加州鲈虹彩病毒病，开展致病机理和免疫防控（口服/浸泡疫苗）研究